Supplemental table for "Adaptation and Validation of the CO₂Balance Method for Ventilation Rate Estimation in Laying Hen Houses"

<p dir="ltr">This study aims to adapt and validate the carbon dioxide (CO₂) balance method for VR prediction in laying hen houses. The adaptation was based on the CO₂ balance method from the International Commission of Agricultural and Biosystems Engineering (CIGR), which considers variables such as the CO₂ production rate of hens (PRCO2), animal activity (AA), and the respiratory quotient of laying hens (RQ).</p><p dir="ltr">The resulting adaptations included setting AA to 1, RQ to 0.9 during the day and 0.85 at night, and PRCO2 to 18% during the day and 8% at night. The adjusted model demonstrated an R² of 0.63 for VR prediction when the difference between exhaled and inlet CO₂ was less than 150 ppm. At the experimental level, all evaluated housing systems showed an R² greater than 0.63 and an average RMSE of 0.35 m³ h^-1 hen^-1. At the commercial level, housing systems exhibited an average predicted R² of 0.71 and an RMSE of 1.68 m³ h^-1 hen^-1. The adapted CO₂ balance method presented good predictive values across laboratory and commercial experiments.</p>