Supplemental information for "Transport of Antibiotics, Antibiotic Resistant Bacteria, and Antibiotic Resistance Genes Through Prairie Strips During Simulated Runoff"

This study evaluated the ability of prairie strips to reduce of three resistance indicators (antibiotics, antibiotic resistant bacteria, and antibiotic resistance genes) from manure impacted overland flow under varying run-on flow rates. High, medium, and low run-on flow rates were tested with the use of a laboratory-scale flume containing a field extracted section of prairie strip. Grab samples were collected immediately upstream, downstream, and beneath the prairie strip representing run-on, runoff, and infiltrated flows, respectively. Percent reductions in the median concentration of each resistance contaminant were calculated for each flow experiment at both the runoff and infiltration sampling locations to demonstrate the impact of the prairie strip. Findings showed that subsurface filtration caused the highest concentration reduction of resistance contaminants. This document includes supplemental tables detailing the number of samples collected throughout each experiment, a complete list of the analytes included in the manure antibiotic analysis, LC-MS/MS method parameters and limits of detection, and qPCR primer sequences and limits of quantification. Additionally, a supplemental figure details the mean concentrations of antibiotic analytes found in the swine manure source during preliminary analysis. A detailed description of the methods used for the antibiotic analysis and quantitative PCR of the manure and water samples is also provided.